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Quantitative gas chromatographic mass spectrometric determination of 1,2,3,4‐tetrahydro‐β‐carboline in human plasma and platelets
Author(s) -
Kari Ilkka,
Peura Pekka,
Airaksinen Mauno M.
Publication year - 1980
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200071120
Subject(s) - chemistry , mass spectrometry , platelet , chromatography , selected ion monitoring , gas chromatography , ion , mass spectrum , beta (programming language) , derivative (finance) , plasma , gas chromatography–mass spectrometry , analytical chemistry (journal) , medicine , physics , organic chemistry , quantum mechanics , computer science , financial economics , economics , programming language
A sensitive and specific method is described for the determination of 1,2,3,4‐tetrahydro‐β‐carboline in human plasma, based on selected ion monitoring gas chromatography mass spectrometry and employing a deuterium analogue as internal standard. The identification of 1,2,3,4‐tetrahydro‐β‐carboline is based on monitoring ions m/z 143, 171,199 and 368 for the heptafluorobutyryl derivative of 1,2,3,4‐tetrahydro‐β‐carboline or the normal mass spectrum. For quantitation the ratio of ions m/z 368/370 was used. The mean of 1,2,3,4‐tetrahydro‐β‐carboline level in human plasma ( n = 10) was 8.78± 1.94 ng ml −1 and 5.68±1.34 ng in platelets corresponding 1 ml of platelet rich plasma.