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Quantitative determination of 6‐Oxo‐PGF 1α in biological fluids by gas chromatography mass spectrometry
Author(s) -
Claeys M.,
van Hove C.,
Duchateau A.,
Herman A. G.
Publication year - 1980
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200071119
Subject(s) - derivatization , chemistry , chromatography , trimethylsilyl , mass spectrometry , selected ion monitoring , gas chromatography , ether , extraction (chemistry) , gas chromatography–mass spectrometry , electron ionization , quantitative analysis (chemistry) , ion , organic chemistry , ionization
A selected ion monitoring method for the determination of 6‐oxo‐PGF 1α , the stable end‐product of prostacyclin, in biological fluids has been developed. In this method, biosynthetically prepared [ 2 H 6 ]‐6‐oxo‐PGF 1α is used as internal standard. The method involves extraction, thin‐layer chromatography purification and derivatization into the methyl ester, methoxime, trimethylsilyl ether derivatives by carrying out the methoximation first. Quantitative gas chromatographic mass spectrometric analysis is performed in the electron impact mode by monitoring the [M – (TMSOH + CH 3 O . )] +. fragment ions. The use of this method in the measurement of 6‐oxo‐PGF 1α in serous fluids and in incubation media of serous tissues is described.