Simultaneous determination of bestatin and  p ‐hydroxybestatin, a major metabolite, in human serum by gas chromatography mass spectrometry. Utilization of quantitative selected ion monitoring and deuterium labelled internal standard | Zendy

Koyama Michinori | Zendy; Hashimoto Mariko | Zendy; Asakawa Noriko | Zendy; Ishibashi Masataka | Zendy; Miyazaki Hiroshi | Zendy

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Simultaneous determination of bestatin and p ‐hydroxybestatin, a major metabolite, in human serum by gas chromatography mass spectrometry. Utilization of quantitative selected ion monitoring and deuterium labelled internal standard

Author(s) -

Koyama Michinori,

Hashimoto Mariko,

Asakawa Noriko,

Ishibashi Masataka,

Miyazaki Hiroshi

Publication year - 1980

Publication title -

biomedical mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0306-042X

DOI - 10.1002/bms.1200070903

Subject(s) - metabolite , chemistry , chromatography , trimethylsilyl , selected ion monitoring , mass spectrometry , detection limit , deuterium , gas chromatography–mass spectrometry , gas chromatography , biochemistry , organic chemistry , physics , quantum mechanics

A specific and sensitive method for the simultaneous determination of bestatin and its major metabolite, p ‐hydroxybestatin in human serum has been developed by selected ion monitoring using the corresponding deuterated internal standards. The N ‐trifluoroacetamide‐ O ‐trimethylsilyl ether derivatives of the trideuteromethyl esters of bestatin and p ‐hydroxybestatin were suitable for quantitation by selected ion monitoring. The determination limit of these compounds by the present method is 1 ng ml −1 in human serum.

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