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Simultaneous determination of bestatin and p ‐hydroxybestatin, a major metabolite, in human serum by gas chromatography mass spectrometry. Utilization of quantitative selected ion monitoring and deuterium labelled internal standard
Author(s) -
Koyama Michinori,
Hashimoto Mariko,
Asakawa Noriko,
Ishibashi Masataka,
Miyazaki Hiroshi
Publication year - 1980
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200070903
Subject(s) - metabolite , chemistry , chromatography , trimethylsilyl , selected ion monitoring , mass spectrometry , detection limit , deuterium , gas chromatography–mass spectrometry , gas chromatography , biochemistry , organic chemistry , physics , quantum mechanics
Abstract A specific and sensitive method for the simultaneous determination of bestatin and its major metabolite, p ‐hydroxybestatin in human serum has been developed by selected ion monitoring using the corresponding deuterated internal standards. The N ‐trifluoroacetamide‐ O ‐trimethylsilyl ether derivatives of the trideuteromethyl esters of bestatin and p ‐hydroxybestatin were suitable for quantitation by selected ion monitoring. The determination limit of these compounds by the present method is 1 ng ml −1 in human serum.