An application of gas chromatography mass spectrometry to the quantitation of substances and their labelled variants | Zendy

Schramm W. | Zendy; Louton T. | Zendy; Schill W. | Zendy; Hoppen H.O. | Zendy

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An application of gas chromatography mass spectrometry to the quantitation of substances and their labelled variants

Author(s) -

Schramm W.,

Louton T.,

Schill W.,

Hoppen H.O.

Publication year - 1980

Publication title -

biomedical mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0306-042X

DOI - 10.1002/bms.1200070610

Subject(s) - chromatography , chemistry , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , selected ion monitoring , isotope , physics , quantum mechanics

A method is proposed for the quantitative estimation of a substance and its labelled analogue using the selected ion monitoring technique of gas chromatography mass spectrometry, in which estimation is achieved without a third labelled analogue as internal standard. In a first measurement the ratio of natural to labelled substance is estabolished. Then a known amount of either labelled or natural compound is added, the isotope ratio is determined a second time and from these two measurements the mass amounts can be calculated. When applied to the heptafluorobutyrates of androstenedione and progesterone about 10 pg of 14 C labeled compounds can be determined in the presence of 1 ng of the natural substances.

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