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An application of gas chromatography mass spectrometry to the quantitation of substances and their labelled variants
Author(s) -
Schramm W.,
Louton T.,
Schill W.,
Hoppen H.O.
Publication year - 1980
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200070610
Subject(s) - chromatography , chemistry , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , selected ion monitoring , isotope , physics , quantum mechanics
A method is proposed for the quantitative estimation of a substance and its labelled analogue using the selected ion monitoring technique of gas chromatography mass spectrometry, in which estimation is achieved without a third labelled analogue as internal standard. In a first measurement the ratio of natural to labelled substance is estabolished. Then a known amount of either labelled or natural compound is added, the isotope ratio is determined a second time and from these two measurements the mass amounts can be calculated. When applied to the heptafluorobutyrates of androstenedione and progesterone about 10 pg of 14 C labeled compounds can be determined in the presence of 1 ng of the natural substances.