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Determination of terbutaline in plasma by gas chromatography chemical ionization mass spectrometry
Author(s) -
Jacobsson SvenErik,
Jönsson Sven,
Lindberg Claes,
Svensson LeifÅke
Publication year - 1980
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200070608
Subject(s) - chemistry , chromatography , mass spectrometry , gas chromatography , reagent , chemical ionization , terbutaline , silylation , coefficient of variation , extraction (chemistry) , analytical chemistry (journal) , ion , ionization , organic chemistry , catalysis , medicine , asthma
A method is described for the determination of unconjugated terbutaline in plasma. The assay is based on gas chromatography chemical ionization mass spectrometry with ammonia as the reagent gas. Terbutaline and a deuterium labelled internal standard were isolated from plasma by ion exchange chromatography followed by extraction into t ‐butanol. Quantitation was performed by selected ion monitoring of the [MH] + ions m/z 442 and m/z 448 of the tri‐O‐TMS derivatives of terbutaline and the internal standard, respectively. Silylation was carried out in the presence of pyridine at room temperature and was complete within one minute. The overall recovery of terbutaline in the procedure was estimated at about 80%. Measurement of terbutaline down to 0.1 ng ml −1 in 4 ml of plasma is possible with a coefficient of variation of 8.6%. Day‐to‐day variation was found to be 6.0% at a level of 3.26 ng ml −1 .