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5‐methoxytryptamine in rat hypothalamus and human CSF. A fact or artifact?
Author(s) -
Narasimhachari N.,
Kempster E.,
Anbar M.
Publication year - 1980
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200070602
Subject(s) - derivatization , hypothalamus , mass spectrometry , chemistry , melatonin , chromatography , gas chromatography–mass spectrometry , thin layer chromatography , gas chromatography , sedative , endocrinology , biology , pharmacology
Using thin‐layer chromatography fluorometry, gas chromatography mass spectrometry and field ionization mass spectrometry the findings of Green et al . regarding the identification of 5‐methoxytryptamine in rat hypothalamus could not be confirmed. On derivatization using pentafluoropropionic anhydride, melatonin underwent partial transacylation to yield 5‐methoxytryptamine as one of the by‐products. We thus consider the finding of 5‐methoxytryptamine in rat hypothalamus by mass fragmentography as an artifact forming from melatonin, which is normally present in rat hypothalamus.