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Analysis of fluorouracil in human plasma by combined gas‐liquid chromatography mass spectrometry
Author(s) -
CosynsDuyck MarieClaire,
Cruyl Annie A. M.,
De Leenheer André P.,
De Schryver André,
Huys John V.,
Belpaire Frans M.
Publication year - 1980
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200070205
Subject(s) - chromatography , chemistry , mass spectrometry , dichloromethane , gas chromatography , extraction (chemistry) , gas chromatography–mass spectrometry , derivative (finance) , selected ion monitoring , high performance liquid chromatography , organic chemistry , solvent , financial economics , economics
Abstract Fluorouracil in human plasma at a concentration as low as 2 ng ml −1 can be assayed with the use of gas‐liquid chromatography and a multiple ion detector. Fluorouracil is isolated from plasma by anion exchange extraction. The extract is butylated and the derivative obtained extracted into a mixture of cyclohexane + dichloromethane (95:5). An aliquot is submitted to combined gas‐liquid chromatography mass spectrometry. The use of chlorouracil or oxygen‐18 labelled flurouracil as internal standard is discussed. Linearity is proven from 100 μg to 5 ng of fluorouracil per ml plasma. The coefficients of variation are 7.2 and 8.4% for within‐run and between‐run precision, respectively, at the 50 ng ml −1 level. The biological applicability of our procedure is demonstrated by analysing plasma samples obtained at different time intervals from three patients given 1 g of fluorouracil once intravenously and once orally.