Premium
Analysis of permethylated glucosaminyl‐glucosaminitol disaccharides by combined gas‐liquid chromatography mass spectrometry
Author(s) -
Jensen Michael,
Borowiak Dietmar,
Paulsen Hans,
Rietschel Ernst Th.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200061205
Subject(s) - disaccharide , chemistry , chromatography , mass spectrometry , glycosidic bond , gas chromatography , glucosamine , anomer , hydrolysis , fast atom bombardment , gas chromatography–mass spectrometry , monosaccharide , high performance liquid chromatography , organic chemistry , enzyme
The α‐and β‐forms of permethylated 1,3‐, 1,4‐ and 1,6‐linked N ‐acetylglucosaminyl‐ N ‐acetylglucosaminitol disaccharides have been analysed directly (without hydrolysis or further modifications) by combined gas‐liquid chromatography mass spectrometry. Gas‐liquid chromatography facilitated the separation of the α‐ and β‐isomers of each disaccharide pair. In every case, the respective α‐form was slower than the β‐form. While, additionally, the α‐ and β‐forms of the 1,6‐linked glucosamine disaccharide could be separated from those of the 1,3‐ and 1,4‐linked disaccharides, the α‐ and β‐forms of the latter two could not be resolved from each other with the liquid phases used. All three disaccharides could be readily differentiated and characterized by mass spectrometry, however. Specific fragments for each glucosamine disaccharide could be defined. Therefore, combined gas‐liquid chromatography mass spectrometry allowed an unequivocal determination of the anomeric configuration and the position of the glycosidic linkage in the glucosamine disaccharides.