Premium
Identification and quantitative determination of o ‐ and m ‐hydroxymandelic acid in human urine
Author(s) -
Midgley John M.,
Couch Margaret W.,
Crowley Jan R.,
Williams Ciyde M.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200061106
Subject(s) - chemistry , urine , mandelic acid , chromatography , creatinine , hydrolysis , acid hydrolysis , enzymatic hydrolysis , biochemistry , organic chemistry
o ‐Hydroxymandelic acid and m ‐hydroxymandelic acid have been identified in human urine by gas chromatography mass spectrometry selected ion monitoring. After solvent extraction the urinary acids were converted to their O ‐trifluoroacetoxy methyl ester derivatives which were identified by comparison of the retention times and relative intensities of the characteristic m/z 374 and m/z 315 ions with those from authentic samples. 4,6‐[ 2 H 2 ]‐ o ‐hydroxymandelic acid and 2,4,6‐[ 2 H 3 ]‐ m ‐hydroxymandelic acid were synthesized for use as internal standards in the quantitative estimation of the isomeric hydroxymandelic acids excreted in urine. In ten normal adults the following results were obtained: o ‐hydroxymandelic acid 4–16 ng mg −1 creatinine and m ‐hydroxymandelic acid 11–71 ng mg −1 creatinine. Acid hydrolysis of the urine or ingestion of a diet of known composition did not affect these results, indicating that these compounds are excreted as the free acids and probably arise by metabolism of the corresponding phenylethanolamine(s).