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Identification of carpronium chloride and its metabolite in human urine by field desorption mass spectrometry using deuterium labelling
Author(s) -
Sano Mitsuji,
Ohya Kazumi,
Ito Ryoji
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200061102
Subject(s) - chemistry , chromatography , mass spectrometry , metabolite , reagent , deuterium , desorption , chloride , urine , ammonium chloride , analytical chemistry (journal) , biochemistry , organic chemistry , physics , quantum mechanics , adsorption
The identification of carpronium chloride and a metabolite in human urine has been performed by means of field desorption mass spectrometry using deuterium labelling. Essential points in this study are the simultaneous administration of a deuterium labelled drug ([ 2 H 9 ]carpronium chloride), a purification procedure by ion pair extraction with an iodine reagent, and the use of paper electrophoresis to examine the degree of clean‐up. The field desorption mass spectra of the purified extracts obtained from sample urine gave a characteristic pattern resulting from the carpronium cation ( m/z 160, m/z 169) and a metabolite of the N ‐(3‐carbohydroxypropyl)trimethyl ammonium cation ( m/z 146, m/z 155).