Premium
The determination of terbutaline in human plasma by selected ion monitoring of the t ‐butyldimethylsilyl ether
Author(s) -
Martin L. E.,
Oxford Janet,
Tanner R. J. N.,
Hetheridge M. J.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200061011
Subject(s) - ether , human plasma , terbutaline , chemistry , chromatography , ion , plasma , environmental chemistry , organic chemistry , physics , medicine , nuclear physics , immunology , asthma
A method is described for the quantitative determination of terbutaline in 2 ml human plasma. The drug is extracted from plasma as the terbutaline tetraphenylboron ion pair and determined by gas chromatography mass spectrometry of its t ‐butyldimethylsilyl ether. Salbutamol is used as internal standard. Quantification is achieved by selected ion monitoring of the ion m/z 482 derived from t ‐butyldimethylsilyl terbutaline and m/z 495 from t ‐butyldimethylsilyl salbutamol. The detection limit was estimated to be 250 pg terbutaline ml −1 plasma. The coefficient of variation at the level of 1 ng terbutaline ml −1 was 4.1% ( n = 5).