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A simple, rapid selected ion monitoring method for the determination of prostaglandins E 2 and F 2α in human urine
Author(s) -
Ferretti Aldo,
Flanagan Vincent P.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200061005
Subject(s) - urine , chromatography , chemistry , coefficient of variation , selected ion monitoring , mass spectrometry , gas chromatography , urinary system , gas chromatography–mass spectrometry , endocrinology , biochemistry , biology
A new procedure for the simultaneous measurement of prostaglandins E 2 and F 2α in human urine is described. Quantification was achieved by gas chromatography mass spectrometry with selected ion monitoring and deuterium labeled internal standards. The levels of measured prostaglandins ranged from 25 pg ml −1 of urine (PGE 2 ) to 2500 pg ml −1 (PGF 2α ). The interassay coefficient of variation, determined by replicate analysis of four identical samples, was 1.9% for PGE 2 and 0.8% for PGF 2α . The procedure takes a fraction of the time needed with published methods and can be conducted with as little as one‐tenth of the daily urinary output.