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Analysis of tyrosine and deuterium labelled tyrosine in tissues and body fluids
Author(s) -
Sjöquist Birgitta
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060905
Subject(s) - tyrosine , amberlite , chemistry , chromatography , deuterium , amino acid , ion exchange , biochemistry , ion , organic chemistry , physics , adsorption , quantum mechanics
A gas chromatographic mass spectrometric method has been developed to determine tyrosine and deuterium labelled tyrosine in biological samples using α‐methyltyrosine (α‐Me Ty) or m ‐hydroxyphenylalanine as internal standards. With the latter standard both labelled and unlabelled tyrosine as well as α‐Me Ty can be determined simultaneously, in for example human blood, following administration of α‐Me Ty to inhibit catecholamine synthesis. After isolation of the amino acids on an ion exchange column (Amberlite IR 120) the butyl ester pentafluoropropionyl derivatives were prepared. In human plasma the precision of the method was determined at a level of 80 nmol tyrosine ml −1 and found to be ±5% (coefficient of variation, n = 10).