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Ion cluster techniques in drug metabolism: An example of the use of mixtures of the [ 12 C]:[ 14 C] isotopic forms of the synthetic prostaglandin cloprostenol (‘Estrumate’) to Facilitate Metabolite Identification
Author(s) -
Bourne G. R.,
Moss S. R.,
Phillips P. J.,
Webster J. T. A.,
White D. F.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060811
Subject(s) - isotope , chemistry , metabolite , stable isotope ratio , natural abundance , abundance (ecology) , kinetic isotope effect , prostaglandin , chromatography , radiochemistry , metabolism , mass spectrometry , cluster (spacecraft) , ion , deuterium , biochemistry , organic chemistry , biology , physics , nuclear physics , computer science , programming language , fishery
The synthetic prostaglandin analogue cloprostenol has been prepared radiolabelled with 14 C. The isotopic abundance of 14 C at position C‐15 was greater than 90% of the theoretical maximum. We have utilized the high abundance of the 14 C isotope for metabolism studies by preparing mixtures of [ 12 C]:[ 14 C]cloprostenol such that fragments detected by mass spectrometry contained characteristic isotope clusters analogous to those often obtained using stable isotopes.