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Identification of urinary metabolites of flavanone in the rat
Author(s) -
Buset Helge,
Scheline Ronald R.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060509
Subject(s) - flavanone , hydroxylation , chemistry , chromatography , metabolite , urine , stereochemistry , biochemistry , enzyme , flavonoid , antioxidant
The urinary metabolites of flavanone in the rat were investigated using a gas chromatographic mass spectrometric method employing an OV‐1 capillary column. Forty‐three metabolites were detected and the most prominent of these were identified. The most common metabolic reactions were reduction of the keto group and hydroxylation at the 3‐ or 6‐positions. Little hydroxylation occurred in ring B. The major metabolites were flavan‐4α‐ol, trans‐3‐hydroxyflavan‐4β‐ol, 6‐hydroxyflavanone and 6‐hydroxyflavan‐4β‐ol. Gas chromatographic analysis of underivatized flavanone metabolites, especially with stainless steel columns, results in the formation of dehydrated products including flavone, flav‐3‐ene and flavanone itself.