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Mass spectrometry of some ultraviolet absorbing derivatives of sugars and related alditols: Identification in biologic fluids after separation by high performance liquid chromatography
Author(s) -
Thompson Richard M.,
Cory David A.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060308
Subject(s) - chemistry , mass spectrum , ion , mass spectrometry , yield (engineering) , chromatography , polyatomic ion , benzoates , chromatographic separation , fractionation , molecular mass , ultraviolet , spectral line , analytical chemistry (journal) , high performance liquid chromatography , organic chemistry , materials science , physics , optoelectronics , metallurgy , enzyme , astronomy
The 70 eV electron impact mass spectra of the acyclic perbenzoyl O ‐benzyloximes of several mono‐ and disaccharides of clinical interest and the perbenzoates of certain related alditols have been obtained and compared with those of other similar derivatives studied previously. In general, the spectra of the benzyloximes contain molecular ions and characteristic fragments due to [C 6 H 5 ] + , [C 7 H 7 ] + , [C 6 H 5 CO] + and [C 6 H 5 COOH] + ˙, losses of HCHO, C 6 H 5 COO. and C 6 H 5 COOH, and cleavages along the carbon chain. The most unique ions in these spectra arise from the combined loss of C 7 H 7 . and C 6 H 5 COO. from the molecular ion: m/z 191 (C 3 ), m/z 339 (deoxy‐C 5 ), m/z 459 (C 5 ), m/z 473 (deoxy‐C 6 ) and m/z 593 (C 6 ). The alditol benzoates fragment similarly, but they do not yield molecular ions. These data are being used to help confirm the identities of carbohydrates in biologic fluids after fractionation by high performance liquid chromatography, prior to the establishment of routine quantitative assays for several of these compounds.