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The determination of testosterone in hamster prostate by gas chromatography mass spectrometry with selected metastable peak monitoring
Author(s) -
Gaskell Simon J.,
Finney Robert W.,
Harper Maureen E.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060307
Subject(s) - mass spectrometry , chromatography , chemistry , gas chromatography , detection limit , resolution (logic) , gas chromatography–mass spectrometry , ion mobility spectrometry , selected ion monitoring , epitestosterone , fragmentation (computing) , analytical chemistry (journal) , androgen , biochemistry , artificial intelligence , computer science , hormone , operating system
Quantitative analyses of testosterone, as the methyl oxime t ‐butyldimethylsilyl ether, are performed by gas chromatography mass spectrometry with selected monitoring of the metastable peak corresponding to the fragmentation [M] + ˙→[M − C 4 ] + in the field free region preceding the electric sector of a double focusing mass spectrometer. A detection limit of c 30 pg is observed during analyses of the standard compound. The method is applied to the quantitative determination of testosterone in extracts of prostatic tissue from the golden hamster, using epitestosterone as the internal standard. The analytical specificity is similar to that achieved during gas chromatography high resolution mass spectrometry with selected ion detection of [M] + ˙ ions; gas chromatography low resolution mass spectrometry is of inadequate specificity.