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Analysis of the venom of the sydney funnel‐web spider, Atrax robustus using gas chromatography mass spectrometry
Author(s) -
Duffield Patricia H.,
Duffield Alan M.,
Carroll Peter R.,
Morgans David
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060305
Subject(s) - chromatography , chemistry , mass spectrometry , venom , gas chromatography , octopamine (neurotransmitter) , eugenol , thermospray , chemical ionization , biochemistry , organic chemistry , tandem mass spectrometry , selected reaction monitoring , ion , receptor , serotonin , ionization
Thirteen compounds have been identified using gas chromatography mass spectrometry in the venom of the Sydney funnel‐web spider, Atrax robustus. The compounds were identified as their trimethylsilyl or pentafluoropropionate derivatives and were citric acid, lactic acid, phosphoric acid, glycerol, urea, glucose, γ‐aminobutyric acid, glycine, spermidine, spermidine, tyramine and octopamine. Female venom contained trace quantities of 5‐methyoxytryptamine which was not detected in male venom. Quantitative determination of tyramine and octopamine was achieved using chemical ionization (CH 4 ) gas chromatography mass spectrometry and deuterated internal standards.