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Quantitation of plasma warfarin levels by gas chromatography chemical ionization mass spectrometry
Author(s) -
Duffield Patricia H.,
Birket D. J.,
Wade D. N.,
Duffield Alan M.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060304
Subject(s) - warfarin , diazomethane , chemistry , chromatography , mass spectrometry , chemical ionization , gas chromatography , gas chromatography–mass spectrometry , ionization , ion , organic chemistry , medicine , atrial fibrillation , cardiology
A quantitative assay for plasma concentrations of warfarin has been developed using a deuterated warfarin analogue as internal standard. The method employs methylation (diazomethane) of warfarin to its 4′‐methoxy derivative and gas chromatography chemical ionization mass spectrometry. Selected ion monitoring was used to relate the response of warfarin methyl ether and the internal standard. This technique can measure warfarin plasma concentrations five days after a single oral (25 mg) dose. Warfarin half‐lives were determined in 12 healthy male volunteers. Each plasma analysis required six minutes of instrument time.