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The analysis of terbutaline in biological fluids by gas chromatography electron impact mass spectrometry
Author(s) -
Clare Roy A.,
Davies Donald S.,
Baillie Thomas A.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060108
Subject(s) - chromatography , chemistry , terbutaline , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , detection limit , selected ion monitoring , extraction (chemistry) , medicine , asthma
A highly sensitive and specific assay is described for the bronchodilator drug terbutaline in human plasma and urine, based on single ion monitoring gas chromatography mass spectrometry and employing a homologue of the drug as internal standard. Recovery of terbutaline and internal standard into ethyl acetate is effected at pH 9.8, while back‐extraction into dilute acid serves to purify the initial extract. Following preparation of O ‐TMS, N ‐TFA derivatives, the drug and its homologue are detected by selected ion monitoring of their common base ion at m/e 355. The limit of detection of terbutaline by this procedure is 0.3 ng ml −1 from a 4 ml sample of plasma.