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Active hydrogen by chemical ionization mass spectrometry
Author(s) -
Lin Yong Yeng,
Smith Leland L.
Publication year - 1979
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200060104
Subject(s) - chemistry , adduct , chemical ionization , mass spectrometry , reagent , hydrogen , protonation , ammonia , ionization , deuterium , ammonium , ion , inorganic chemistry , organic chemistry , chromatography , physics , quantum mechanics
A means of determination of active hydrogen of hydroxyl, carboxyl, sulfhydryl, amino, amido and sulfonamido groups by chemical ionization mass spectrometry using ammonia and trideuterioammonia as reagent gases is described. The method is based on exchange of active hydrogen for deuterium occurring during the chemical ionization process using trideuterioammonia, with comparison of m/z for ammonium adduct [M+NH 4 ] + and trideuterioammonium adduct [M‐nH+nD+ND 4 ] + , or of protonated molecular ion [M+H] + and deuteronated molecular ion [M‐nH+nD+D] + yielding the number of active hydrogens. Applications have been made to several classes of biologically important compounds.