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Determination of clonazepam and its amino and acetamido metabolites in human plasma by combined gas chromatography chemical ionization mass spectrometry and selected ion monitoring
Author(s) -
Min B. H.,
Garland W. A.,
Khoo K.C.,
Torres G. S.
Publication year - 1978
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200051211
Subject(s) - metabolite , chemistry , chromatography , isotope dilution , mass spectrometry , chemical ionization , gas chromatography–mass spectrometry , clonazepam , gas chromatography , amine gas treating , ionization , ion , organic chemistry , biochemistry , psychology , psychiatry
Abstract Our previously published gas chromatography chemical ionization mass spectrometry stable isotope dilution assay for clonazepam and its 7‐amino metabolite in plasma has been modified to obtain more consistent recoveries of the amine metabolite and to permit the determination of the 7‐acetamido metabolite of clonazepam. In addition, the manuscript describes the details of a method for a least squares analysis of the nonlinear standard curves associated with the use of certain stable isotope labeled analogs as internal standard.