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Quantitative analysis of propranolol and metabolites by a gas chromatograph mass spectrometer computer technique
Author(s) -
Vu Van T.,
Abramson Fred P.
Publication year - 1978
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200051210
Subject(s) - chromatography , chemistry , amberlite , mass spectrometry , reproducibility , gas chromatography , gas chromatography–mass spectrometry , urine , selected ion monitoring , propranolol , quantitative analysis (chemistry) , hydrolysis , organic chemistry , biochemistry , adsorption , medicine
A quantitative assay for urinary propranolol and six of its unconjugated metabolites by a gas chromatography mass spectrometry computer system has been developed. Conjugated metabolites are also determined following enzyme hydrolysis. These compounds are separated from urine by Amberlite XAD‐2 resin column chromatography and are converted to methyl esters and trifuoroacetyl derivatives. Each substance is quantitated from reconstructed ion chromatograms using a specific mass or sum of masses for each compound by comparing its area with that of an appropriate internal standard. The minimum detectable concentrations are in the range of 50–100 ng ml −1 . The calibrations are linear from 0.10–15.0 μg ml −1 with recoveries ranging from 62–91%. This procedure yields a high degree of specificity, selectivity and reproducibility, as demonstrated by the analyses of urine from dogs receiving single 40 mg doses of propranolol orally.