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Quantitative assay of the N ‐methylated metabolites of tryptamine and serotonin by gas chromatography mass spectrometry as applied to the determination of lung indoleethylamine N ‐methyltransferase activity
Author(s) -
Räisänen M.,
Kärkkäinen J.
Publication year - 1978
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200051010
Subject(s) - tryptamine , chemistry , chromatography , mass spectrometry , gas chromatography , serotonin , gas chromatography–mass spectrometry , methyltransferase , quantitative analysis (chemistry) , biochemistry , methylation , receptor , gene
A specific and sensitive method is described for the identification and quantification of the N ‐mono‐ and dimethylated derivatives of tryptamine and serotonin by gas chromatography mass spectrometry. Deuterated analogues of the amines have been prepared for use as internal standards. The technique has been applied to the determination of indoleethylamine N ‐methyltransferase activity in rabbit and human lung. No interference from the β‐carboline formation or other side reactions between the substrates and the methyl donor was observed.