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Determination of choline and acetylcholine in distinct rat brain regions by stable isotope dilution and field desorption mass spectrometry
Author(s) -
Lehmann W. D.,
Schulten H.R.,
Schröder N.
Publication year - 1978
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200051009
Subject(s) - isotope dilution , chemistry , desorption , mass spectrometry , chromatography , field desorption , derivatization , isotope , acetylcholine , analytical chemistry (journal) , extraction (chemistry) , choline , ion , ionization , biochemistry , physics , organic chemistry , adsorption , quantum mechanics , medicine , endocrinology
The determination of choline and acetylcholine by field desorption mass spectrometry from rat brain tissue samples has been demonstrated. Essential points of the assay are the use of stable isotope labelled internal standards, a simple ion pair extraction procedure, direct analysis of the quaternary ammonium ions without derivatization, and accumulation of the electrically recorded field desorption ion signals with a multi‐channel analyser. The analysis of sample amounts in the picogram range gave quantitative data of good precision (0.6–10% standard deviation).