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Use of a new silylating agent for separation of bile acids and cholesterol by selected ion monitoring with the computer controlled intensity matching technique
Author(s) -
Miyazaki Hiroshi,
Ishibashi Masataka,
Yamashita Kouwa
Publication year - 1978
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200050802
Subject(s) - lithocholic acid , chemistry , chromatography , bile acid , elution , ether , selected ion monitoring , mass spectrometry , gas chromatography , gas chromatography–mass spectrometry , organic chemistry , biochemistry
Dimethylethylsilyl ether derivatives of human biliary bile acid ethyl esters and cholesterol were separated completely by gas chromatography and selected ion monitoring by use of the mixed stationary liquid phase of OV‐101 and Dexsil 300GC (1:1). These derivatives could be prepared easily and subjected directly to gas chromatography mass spectrometry. Dimethylethylsilyl ethers of bile acids ethyl esters were eluted in regular order according to the number of hydroxyl groups when used with the non‐polar stationary liquid phases. Lithocholic acid, a minor component of human bile, was identified easily by selected ion monitoring with the computer controlled intensity matching technique.