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Development of a procedure for the assay of an experimental steroid drug in dog plasma
Author(s) -
Gaskell Simon J.,
Brooks Charles J. W.,
Matin Shaikh B.
Publication year - 1978
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200050708
Subject(s) - steroid , drug , pharmacology , plasma , chemistry , medicine , hormone , physics , quantum mechanics
Abstract Studies of the estimation of 16α‐cyano‐3β‐cyclopentyloxypregn‐5‐en‐20‐one (an experimental drug) in dog plasma are described. Extraction using a salt/solvent pair (ammonium carbonate/ethyl acetate) is followed by a rapid chromatographic procedure employing Lipidex 5000, which affords a substantially purified fraction. After preparation of the t ‐butyldimethylsilyloxime, quantification of the drug is performed by selected ion monitoring. The [ 2 H 9 ]cyclopentyloxy analogue is used as an internal standard. In a preliminary experiment, the advantages (in terms of both sensitivity and selectivity) of the use of an open tubular GLC column are demonstrated.