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Urinary excretion of 5β‐pregnane‐3α,20α,21‐triol in human gestation
Author(s) -
Begue RenéJean,
Moriniere Madeleine,
Padieu Prudent
Publication year - 1978
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200050304
Subject(s) - pregnane , chemistry , pregnanediol , triol , urine , chromatography , urinary system , endocrinology , medicine , stereochemistry , biochemistry , organic chemistry , diol
The steroids in urine from normal pregnant women have been studied. After extraction of conjugate steroids, solvolysis and enzymatic hydrolysis, the liberated steroids were separated by chromatography on Sephadex LH‐20, and were analysed by gas‐liquid chromatography and gas chromatography mass spectrometry. The following steroids were isolated and completely identified in the LH‐20 fraction 7: 5β‐pregnane‐3α,20α‐diol, 5β‐pregnane‐3α,17,20α‐triol, 5α‐pregnane‐3α,20α,21‐triol and 5α‐pregnane‐3β,16α,20α‐triol. In addition, two metabolites tentatively identified as 5ξ‐pregnane‐2ξ,3ξ,20ξ‐triol and 2ξ,3ξ,16ξ‐trihydroxy‐5ξ‐pregnan‐20‐one, have not been reported as occurring in urine from pregnant women. The 5β‐pregnane‐3α,20α,21‐triol was detected only in the third trimester of pregnancy and the urinary excretion values are between 320 and 650 μg per 24 h. With the present data, it is not possible to establish the precursor(s) of this steroid. However, these results tentatively suggest that 5β‐pregnane‐3α,20α,21‐triol arises from foeto‐placental unit.