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Gas chromatography chemical ionization mass spectrometric analysis of diminazene in plasma
Author(s) -
Fouda Hassan G.
Publication year - 1978
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200050113
Subject(s) - chemistry , chromatography , detection limit , chemical ionization , mass spectrometry , gas chromatography , plasma , ionization , ion , organic chemistry , physics , quantum mechanics
A quantitative and sensitive method was developed for the determination of diminazene in plasma. The assay involves the reduction of diminazene to 4‐aminobenzamidine and 4‐hydrazinobenzamidine. The latter is further reduced to give an additional mole of 4‐aminobenzamidine which is extracted, acetylated and condensed with hexafluoroacetylacetone to form a volatile derivative that is subsequently analyzed by gas chromatography chemical ionization mass spectrometry. 4‐Aminobenzylamidine was synthesized and used as an internal standard. The method is reproducible and its sensitivity limit using 1 ml of plasma is 0.1 μg diminazene ml −1 . This sensitivity limit is sufficient to detect plasma levels in cattle following therapeutic doses of the drug.