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Gas chromatography mass spectrometry assay for ketamine and its metabolites in plasma
Author(s) -
Lau Serrine S.,
Domino Edward F.
Publication year - 1977
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200040508
Subject(s) - chromatography , chemistry , mass spectrometry , gas chromatography , ketamine , reproducibility , electron ionization , gas chromatography–mass spectrometry , selected ion monitoring , plasma , ionization , ion , medicine , organic chemistry , anesthesia , physics , quantum mechanics
A highly sensitive and specific method for quantitation of ketamine and two of its metabolites found in monkey, dog and human plasma was developed using a gas chromatography selected ion monitoring assay operated in both electron impact and chemical ionization modes. This technique yields excellent precision, reproducibility and accuracy. From 1 ng to 3 μg of ketamine or its metabolites in 1 ml of plasma can be detected. The technique of electron capture gas chromatography is compared. All three assays were found to be quite useful but the gas chromatography mass spectrometry techniques were more specific.