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Analysis of drugs by pyrolysis. I ‐selected ion monitoring combined with a pyrolysis method for the determination of carpronium chloride in biological samples
Author(s) -
Ohya Kazumi,
Sano Mitsuji
Publication year - 1977
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200040410
Subject(s) - pyrolysis , chloride , chemistry , hydrogen chloride , ion , nuclear chemistry , inorganic chemistry , organic chemistry
In order to establish an analytical method for carpronium chloride, a parasympathomimetic agent, the pyrolysis reaction of carpronium chloride was examined in a g.c.m.s. system, which revealed that γ‐butyrolactone was produced directly from the drug as the main pyrolysis product. In the case of conversion of [2,2,3,3‐ 2 H 4 ]carpronium chloride into the deuterated γ‐butyrolactone, 2 H/ 1 H scrambling was observed and confirmed to occur during the pyrolysis process of the deuterated compound. The proportion of γ‐[ 2 H 4 ]butyrolactone among the pyrolysis products was almost independent of the operating conditions, so [2,2,3,3‐ 2 H 4 ]carpronium chloride was of practical use as an internal standard for selected ion monitoring. By incorporation of the pyrolysis reaction of carpronium chloride with selected ion monitoring and the use of [2,2,3,3‐ 2 H 4 ]carpronium chloride as an internal standard, a rapid, sensitive and selective method was devised for the determination of the drug in biological samples. The method was utilized successfully for the biopharmaceutical studies of carpronium chloride in man.