Premium
Rapid and quantitative blood amino acid analysis by chemical ionization mass spectrometry
Author(s) -
Mee John M. L.,
Korth John,
Halpern Berthold,
James Lewis B.
Publication year - 1977
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200040310
Subject(s) - mass spectrometry , chromatography , chemistry , chemical ionization , quantitative analysis (chemistry) , amino acid , amino acid analysis , ionization , direct electron ionization liquid chromatography–mass spectrometry interface , phenylalanine , analytical chemistry (journal) , organic chemistry , biochemistry , ion
A quantitative analysis of amino acids in microsamples of dried blood spots by chemical ionization mass spectrometry has been developed. Isotope ratio determination was used as the quantitating technique via multiple labelled internal standards. This procedure yields excellent precision and accuracy as demonstrated by the analysis of known amino acid mixtures and of phenylalanine in the blood from newborns.