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Determination of mass spectrometric sensitivity of different metalloporphyrin esters relative to porphyrin ester
Author(s) -
Larsen Elfinn,
Egsgaard Helge,
Møller Jørgen,
With Torben K.
Publication year - 1977
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200040211
Subject(s) - porphyrin , chemistry , sensitivity (control systems) , chromatography , medicinal chemistry , organic chemistry , engineering , electronic engineering
Quantitative determination of metalloporphyrin contamination in preparations of biologically important porphyrins was achieved mass spectrometrically by application of the integrated ion current technique. For this purpose, the relative molecular ion sensitivities of the contaminating metal complexes were determined from the ratios of the integrated molecular ion currents of a series of calibration samples containing a porphyrin ester and one of its metal complexes in known molar ratio. Complexes formed with divalent ions of Cu, Zn, Fe, Co and Ni of copro ‐ as well as uro ‐prophyrin permethylester were all found to have the same molecular ion sensitivities as their metal‐free porphyrin ester. The relative metalloporphyrin ester content in a sample of porphyrin ester was thus obtained directly as the integrated ion current ratios of the normalized molecular ions. The preparation of calibration samples, including complex formation and the mass spectrometric methodology, is described and discussed.