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Chemical ionization mass spectrometry of polyunsaturated fatty acids of human serum
Author(s) -
Araki Eiji,
Ariga Toshio,
Murata Takeshi
Publication year - 1976
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200030602
Subject(s) - chemistry , chemical ionization , mass spectrometry , polyunsaturated fatty acid , derivatization , electron ionization , chromatography , isobutane , trimethylsilyl , gas chromatography–mass spectrometry , mass spectrum , fast atom bombardment , gas chromatography , reagent , organic chemistry , ionization , fatty acid , ion , catalysis
Abstract Polyunsaturated fatty acids of human serum were analysed by combination gas‐liquid chromatography and chemical ionization mass spectrometry, using isobutane as the reagent gas. The method described yielded the molecular ion of long chain unsaturated fatty acids as the base peak; these were weak or unobservable in the corresponding electron impact mass spectra. In the chemical ionization mass spectromtery, the derivatization with trimethylsilyl ether was demonstrated to be useful for the location of the double bonds of unsaturated fatty acids. Applied to the serum polyunsaturated fatty acids, these methods have given identification of C 18:4 , C 20:4 , C 20:5 , C 22:4 , C 22:5 ω‐6, C 22:5 ω‐3, and C 22:6 .