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Selected ion monitoring assay for the bronchodilator pirbuterol
Author(s) -
Falkener Fred C.,
McIlhenny Hugh M.
Publication year - 1976
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200030503
Subject(s) - bronchodilator , chemistry , potassium carbonate , chromatography , medicine , organic chemistry , asthma
A selected ion monitoring assay has been developed to measure plasma levels of the bronchodilator pirbuterol in animals and man. Pirbuterol is extracted from plasma with acetone + potassium carbonate and after further purification is converted to its trimethylacetyl derivative. With the use of the stable isotope labeled internal standard, pirbuterol‐ d 9 , precisions of 10% and 6% were achieved at plasma concentrations of 1 and 20 ng ml −1 , respectively. The synthesis of the deuterium labeled internal standard is described.