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Qualitative and quantitative mass spectrometry of some biologically important trace metals as their tetraphenylporphyrin chelates
Author(s) -
Davis B. A.,
Hui K. S.,
Durden D. A.,
Boulton A. A.
Publication year - 1976
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200030206
Subject(s) - tetraphenylporphyrin , chemistry , mass spectrometry , chelation , thermal decomposition , deuterium , analytical chemistry (journal) , calibration curve , calibration , trace amounts , decomposition , inorganic chemistry , chromatography , porphyrin , organic chemistry , detection limit , medicine , physics , statistics , mathematics , alternative medicine , quantum mechanics , pathology
The use of the mass spectrometric integrated ion current technique for the quantitative analysis of some trace metals at their tetraphenylporphyrin chelates is described. The linearity of the calibration curves and the sensitivity of the technique are improved by employing deuterated tetraphenylporphyrin chelates as loading agents. Femtomole quantities of the chelates may be detected. The use of deuterated tetraphenylporphyrin chelates as internal standards overcomes the problems of losses occurring during chromatographic separation and by thermal decomposition in the mass spectrometer.