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Derivatization of aminoalkylphosphonic acids for characterization by gas chromatography mass spectrometry
Author(s) -
Rueppel M. L.,
Suba L. A.,
Marvel J. T.
Publication year - 1976
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200030106
Subject(s) - derivatization , trifluoroacetic anhydride , chemistry , mass spectrometry , trifluoroacetic acid , chromatography , gas chromatography , gas chromatography–mass spectrometry , derivative (finance) , fragmentation (computing) , silylation , organic chemistry , computer science , financial economics , economics , catalysis , operating system
A general derivatization method has been developed for both primary and secondary aminoalkylphosphonic acids. Derivatization is effected by reaction with trifluoroacetic acid and trifluoroacetic acid anhydride followed by esterification using ethereal diazo‐ n ‐butane. This method can be used for microgram quantities and permits ready characterization by both gas chromatography and gas chromatography mass spectrometry. The structure of this derivative has been verified by mass spectrometry and proton magnetic resonance. Fragmentation patterns were confirmed by high resolution mass spectrometry, isotope labeling and the formation of derivative analogs.