Premium
Determination of a mannose‐containing trisaccharide in the urine of patients and heterozygotes of mannosidosis
Author(s) -
Lundblad Arne,
Masson Parvesh K.,
Nordén Nils E.,
Svensson Sigfrid,
Öckerman PerArne
Publication year - 1975
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200020602
Subject(s) - trisaccharide , urine , chromatography , chemistry , mass spectrometry , mannose , gas chromatography , biochemistry
A method employing gas‐liquid chromatography and combined gas‐liquid chromatography mass spectrometry was developed for the quantitative determination of α‐D‐mannopyranoside‐(1 → 3)‐β‐D‐mannopyranoside‐(1 → 4)‐2‐acetamido‐2‐deoxy‐D‐glucose in urines. The concentration of this trisaccharide varied between 123 and 469 mg per litre of urine in the patients with mannosidosis but was not detected in twenty normals and two heterozygotes.