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Thin‐layer chromatography and high resolution selected ion monitoring for the analysis of C 19 steroids in human hyperplastic prostate tissue
Author(s) -
Millington D. S.,
Buoy M. E.,
Brooks G.,
Harper M. E.,
Griffiths K.
Publication year - 1975
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200020411
Subject(s) - chemistry , chromatography , resolution (logic) , thin layer chromatography , gas chromatography , mass spectrometry , steroid , selected ion monitoring , dihydrotestosterone , prostate , gas chromatography–mass spectrometry , androgen , medicine , biochemistry , hormone , cancer , artificial intelligence , computer science
Interfering nonpolar lipid material was removed from the acetone extracts of several samples of benign hyperplastic prostate tissue. Endogenous steroids were separated by preparative thin‐layer chromatography into fractions containing testosterone, 5α‐dihydrotestosterone and the 5α‐androstane‐3, 17‐diols, and concentrations of these steroids were measured by molecular ion monitoring of suitable derivatives at a resolution of 8500 to 10 000 during combined gas chromatography mass spectrometry. The sensitivity and specificity of this procedure allowed unequivocal detection of the steroids of interest at the femtomole level and the incorporation of epimers as internal standards enabled accurate quantitative measurements. The results indicated that 5α‐dihydrotestosterone is usually the most predominant of the steroids measured, and that of the 5α‐androstanediols, only the 3α, 17β and 3β, 17β isomers occur in measurable concentration, with the former usually predominant.