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Estimation of picomole quantities of piperidine in tissues
Author(s) -
Seiler N.,
Schneider H. H.
Publication year - 1974
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200010604
Subject(s) - piperidine , derivatization , reagent , chemistry , chromatography , chloride , perchloric acid , organic chemistry , mass spectrometry
Piperidine is a putative neurotransmitter. Its sensitive determination is one of the prerequisites for the evaluation of its assumed physiological role. For its derivatization 5‐di‐ n ‐butylaminonaphthalene‐1‐sulphonyl chloride, a new reagent which improves methods applying 5‐dimethylaminonaphthalene‐1‐sulphonyl chloride, is used. Reaction of the perchloric acid tissue extract with this reagent, thin‐layer chromatographic separation of the fluorescent derivatives and mass spectrometric evaluation of the di‐ n ‐butylaminonaphthalenesulphonyl piperidine spot by a version of the integrated ion current technique allows the determination of piperidine in tissues in picomole quantities. Standard deviation of the determinations of 2 to 16 pmol of piperidine added to 50 mg of brain tissue was ±8%. The method was applied to the analysis of different tissues. Brain concentrations of piperidine in different species were found to be lower by a factor up to 100 in comparison with published data.