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Determination of phenformin in biological fluids using chemical ionization mass spectrometry
Author(s) -
Matin Shaikh B.,
Karam John H.,
Forsham Peter H.,
Knight James B.
Publication year - 1974
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200010505
Subject(s) - phenformin , chemistry , biguanide , trifluoroacetic anhydride , mass spectrometry , chromatography , chemical ionization , organic chemistry , ionization , medicine , ion , insulin , metformin
A method for the quantification of phenformin, an oral hypoglycemic biguanide, from biological fluids based upon gas chromatography and chemical ionization mass spectrometry, is reported. Phenformin is quantitatively cyclized to the 2‐substituted‐4‐trifluoremethyl‐2,6‐diamino‐1,3,5‐ S ‐triazine when acylated with trifluoroacetic anhydride. This cyclized product was measured with respect to the deuterium substituted phenformin. The plasma levels achieved following the administration of a therapeutic dose of phenformin to a diabetic subject are reported.