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Thin‐layer chromatography‐mass spectrometry for the identification of sapogenins from Digitalis species
Author(s) -
Evans F. J.
Publication year - 1974
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200010304
Subject(s) - sapogenin , chromatography , thin layer chromatography , chemistry , steroid , mass spectrometry , thin layer , layer (electronics) , biochemistry , organic chemistry , hormone , pathology , medicine , alternative medicine
A method is described for the identification of the sapogenins of several Digitalis species. The steroids are separated by repeated development thin‐layer chromatography and identified by means of high resolution mass spectrometry. The spectra of tigogenin, gitogenin, digalogenin and digitogenin are recorded. These spectra are of particular use in establishing the position of extra hydroxy moieties in the steroid nuclei. Several species of Digitalis were screened for their sapogenin content. D. purpurea, D. mertonensis and D. lutea were found to contain tigogenin and digitogenin in small amounts, the principal steroid being gitogenin, whilst D. ambigua and D. lanata contained all four steroids.