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Qualitative and quantitative mass spectrometry of some noncatecholic biogenic amines and related compounds as their dansyl derivatives
Author(s) -
Durden D. A.,
Davis B. A.,
Boulton A. A.
Publication year - 1974
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200010202
Subject(s) - chemistry , mass spectrometry , chromatography , fragmentation (computing) , dansyl chloride , sublimation (psychology) , derivatization , psychology , computer science , psychotherapist , operating system
The use of the integrated ion current technique for the analysis of biogenic amines and phenols as their 1‐dimethylaminonaphthalene‐5‐sulphonyl (dansyl) derivatives is described, particularly as regards to the linearity and sensitivity of detection. The mono derivatized amines gave a linear response over the range 10 −9 to 10 −14 mol, with a few being detected in quantities as small as 5 × 10 −15 mol. The bisdansyl derivatives have a lower sensitivity and the response is linear over a more restricted range, terminating at 10 −13 mol. Deuterated analogues of some of the amines were prepared and the feasibility of their use as internal standards was investigated. In this way, the problems associated with quantitative biochemical analyses caused by losses during extractions, chromatographic separations and thermal decomposition in the mass spectrometer lon source are overcome. The differentiation of isomers of biogenic amines as their dansyl derivatives has been accomplished by fractional sublimation and by examination of differences in their fragmentation patterns.