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Simultaneous determination of naringenin and hesperetin in rats after oral administration of Da‐Cheng‐Qi decoction by high‐performance liquid chromatography–tandem mass spectrometry
Author(s) -
Liu Ying,
Xu Fengguo,
Zhang Zunjian,
Song Rui,
Tian Yuan
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.991
Subject(s) - hesperetin , chemistry , chromatography , naringenin , decoction , hesperidin , protein precipitation , atmospheric pressure chemical ionization , high performance liquid chromatography , methanol , mass spectrometry , formic acid , pharmacokinetics , tandem mass spectrometry , electrospray ionization , chemical ionization , flavonoid , pharmacology , ionization , ion , traditional medicine , biochemistry , organic chemistry , medicine , alternative medicine , pathology , antioxidant
To quantify naringenin and hesperetin in rat plasma after oral administration of Da‐Cheng‐Qi decoction, a famous purgative traditional Chinese medicine, a high‐performance liquid chromatography–tandem mass spectrometry method was developed and validated. The HPLC separation was carried out on a Zorbax SB‐C 18 column using 0.1% formic acid–methanol as mobile phase and estazolam as internal standard after the sample of rat plasma had been cleaned up with one‐step protein precipitation using methanol. Atmospheric pressure chemical ionization in the positive ion mode and selected reaction monitoring method was developed to determine the active components. This method was validated in terms of recovery, linearity, accuracy and precision (intra‐ and inter‐batch variation). The recoveries of naringenin and hesperetin were 72.8–76.6 and 75.7–77.2%, respectively. Linearity in rat plasma was observed over the range of 0.5–250 ng/mL ( r 2 > 0.99) for both naringenin and hesperetin. The accuracy and precision were well within the acceptable range and the relative standard deviation of the measured rat plasma samples was less than 15% ( n = 5). The validated method was successfully applied for the evaluation of the pharmacokinetics of naringenin and hesperetin administered to six rats. Copyright © 2008 John Wiley & Sons, Ltd.