Determination of bisphenol A and 10 alkylphenols in serum using SDS micelle capillary electrophoresis with γ‐cyclodextrin

A new micelle capillary electrophoresis based on cyclodextrin micellar electrokinetic chromatography (MEKC) for the determination of bisphenol A and 10 alkylphenols in rat serum is reported. Several surfactants and dextrins were studied. Bisphenol A and alkylphenols were separated using a 50 µm × 50 cm capillary with 20 m M borate phosphate buffer (pH 8.0) containing 20 m M sodium dodecylsulfate and 5 m M γ‐cyclodextrin as carrier. The method could determine 0.6–2000 µg/mL of phenols in 100 µL serum by photometric detection at 214 nm. Using 2.0 mL serum, 1.0 ng/mL of phenols could be determined. The relative standards deviations were 6.3–7.7% at 10 µg/mL in serum. The recoveries were 91.8–93.0% with 10 µg/mL serum samples. Copyright © 2001 John Wiley & Sons, Ltd.