Premium
Determination of bisphenol A and 10 alkylphenols in serum using SDS micelle capillary electrophoresis with γ‐cyclodextrin
Author(s) -
Katayama Masatoki,
Matsuda Yoshifumi,
Sasaki Tadahiro,
Shimokawa Kenichi,
Kaneko Satoru,
Iwamoto Teruaki
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.99
Subject(s) - chemistry , micellar electrokinetic chromatography , chromatography , capillary electrophoresis , bisphenol a , cyclodextrin , phenols , micelle , electrophoresis , aqueous solution , organic chemistry , epoxy
A new micelle capillary electrophoresis based on cyclodextrin micellar electrokinetic chromatography (MEKC) for the determination of bisphenol A and 10 alkylphenols in rat serum is reported. Several surfactants and dextrins were studied. Bisphenol A and alkylphenols were separated using a 50 µm × 50 cm capillary with 20 m M borate phosphate buffer (pH 8.0) containing 20 m M sodium dodecylsulfate and 5 m M γ‐cyclodextrin as carrier. The method could determine 0.6–2000 µg/mL of phenols in 100 µL serum by photometric detection at 214 nm. Using 2.0 mL serum, 1.0 ng/mL of phenols could be determined. The relative standards deviations were 6.3–7.7% at 10 µg/mL in serum. The recoveries were 91.8–93.0% with 10 µg/mL serum samples. Copyright © 2001 John Wiley & Sons, Ltd.