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Simple HPLC‐UV determination of nucleosides and its application to the authentication of Cordyceps and its allies
Author(s) -
Ikeda Rie,
Nishimura Miho,
Sun Yen,
Wada Mitsuhiro,
Nakashima Kenichiro
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.980
Subject(s) - cordycepin , cordyceps , chemistry , uridine , guanosine , cordyceps militaris , deoxyadenosine , chromatography , adenosine , high performance liquid chromatography , gradient elution , biochemistry , food science , rna , gene
A simple HPLC‐UV method combined with a simple extraction procedure of nucleosides (adenosine, cordycepin, 2′‐deoxyadenosine, guanosine and uridine) was developed and applied to the authentication of Cordyceps and its allies. The separation was performed on a C 18 column by isocratic elution with acetonitrile–water, and UV detection at 260 nm. The amounts of adenosine, cordycepin, 2′‐deoxyadenosine, guanosine and uridine in Cordyceps were 0.28–14.15, 0.006–6.36, 0.01–0.14, 0.68–14.79 and 0.19–20.29 mg/g, respectively. Among the nucleosides studied, cordycepin was characteristically included in Cordyceps militaris (L.) Link. (CM), which is one of key Cordyceps allies, and might be a good marker for authenticating CM. The ratio of nucleosides to adenosine contents in Cordyceps seemed to be a useful marker for authentication and quality control of Cordyceps . Copyright © 2008 John Wiley & Sons, Ltd.