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Determination of icariin in rat plasma by reverse‐phase high‐performance liquid chromatography after oral administration of a lipid‐based suspension of Epimedium koreanum extract
Author(s) -
Pozharitskaya Olga N.,
Karlina Marina V.,
Shikov Alexander N.,
Kosman Vera M.,
Makarova Mari.,
Makarov Valery G.
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.978
Subject(s) - icariin , chemistry , chromatography , rutin , detection limit , epimedium , high performance liquid chromatography , extraction (chemistry) , methanol , quantitative analysis (chemistry) , solvent , biochemistry , organic chemistry , traditional medicine , medicine , alternative medicine , herb , pathology , antioxidant , medicinal herbs
A simple, specific and sensitive method for quantitative determination of icariin in rat plasma using reverse‐phase high‐performance liquid chromatography with UV‐detection was developed and applied to an animal study of a lipid‐based suspension of the Epimedium koreanum extract in rats. Rutin was selected as the internal standard and methanol was found to be the best solvent for extraction of icariin from the plasma. Linearity was observed between 0.030 and 100 µg/mL ( r > 0.99). The extraction recoveries of icariin and rutin were approximately 75 and 80%, respectively, in plasma. The intra‐ and inter‐day coefficients of variation were less than 5%. The limit of detection was 6 ng/mL and the limit of quantification was 18 ng/mL. Copyright © 2008 John Wiley & Sons, Ltd.