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Analysis of hairy root culture of Rauvolfia serpentina using direct analysis in real time mass spectrometric technique
Author(s) -
Madhusudanan K. P.,
Banerjee Suchitra,
Khanuja Suman P. S.,
Chattopadhyay Sunil K.
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.974
Subject(s) - chemistry , dart ion source , dart , hairy root culture , reserpine , mass spectrometry , chromatography , organic chemistry , agrobacterium , biochemistry , biology , ion , electron ionization , computer science , transformation (genetics) , programming language , gene , ionization , endocrinology
The applicability of a new mass spectrometric technique, DART (direct analysis in real time) has been studied in the analysis of the hairy root culture of Rauvolfia serpentina . The intact hairy roots were analyzed by holding them in the gap between the DART source and the mass spectrometer for measurements. Two nitrogen‐containing compounds, vomilenine and reserpine, were characterized from the analysis of the hairy roots almost instantaneously. The confirmation of the structures of the identified compounds was made through their accurate molecular formula determinations. This is the first report of the application of DART technique for the characterization of compounds that are expressed in the hairy root cultures of Rauvolfia serpentina . Moreover, this also constitutes the first report of expression of reserpine in the hairy root culture of Rauvolfia serpentina . Copyright © 2008 John Wiley & Sons, Ltd.