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Simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma by liquid chromatography–tandem mass spectrometry: application to a pharmacokinetic study of a standardized fraction of Salvia miltiorrhiza, PF2401‐SF
Author(s) -
Park Eun Jeong,
Ji Hye Young,
Kim Nam Jin,
Song Won Young,
Kim Young Hoon,
Kim YounChul,
Sohn Dong Hwan,
Lee Hye Suk
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.968
Subject(s) - salvia miltiorrhiza , chemistry , chromatography , salvia , angelica sinensis , ammonium formate , selected reaction monitoring , tandem mass spectrometry , mass spectrometry , pharmacokinetics , high performance liquid chromatography , liquid chromatography–mass spectrometry , detection limit , hypericum perforatum , pharmacology , traditional medicine , traditional chinese medicine , medicine , alternative medicine , pathology
A rapid, sensitive and selective liquid chromatography–tandem mass spectrometric (LC‐MS/MS) method for the simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone, the active components of Salvia miltiorrhiza in rat plasma, was developed. After liquid–liquid extraction with tariquidar as an internal standard, tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone were eluted from an Atlantis dC 18 column within 5 min with a mixture of methanol and ammonium formate (10 m m , pH 6.5; 85:15, v/v). The analytes were detected by an electrospray ionization tandem mass spectrometry in the selected reaction monitoring (SRM) mode. The standard curves were linear ( r = 0.999) over the concentration range of 0.25–80 ng/mL for tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma. The coefficients of variation and the relative errors of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone for intra‐ and inter‐assay at four quality control (QC) concentrations were 1.1–5.1% and −4.0–6.0%, respectively. The lower limit of quantification for tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone was 0.25 ng/mL from 100 µL of plasma. This method was successfully applied to the pharmacokinetic study of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone after oral administration of PF2401‐SF, the standardized fraction of Salvia miltiorrhiza enriched with tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone to male Sprague–Dawley rats. Copyright © 2008 John Wiley & Sons, Ltd.