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Simultaneous clinical monitoring of lactic acid, pyruvic acid and ketone bodies in plasma as methoxime/tert‐butyldimethylsilyl derivatives by gas chromatography–mass spectrometry in selected ion monitoring mode
Author(s) -
Paik ManJeong,
Cho EunYoung,
Kim Hoon,
Kim KyoungRae,
Choi Sangdun,
Ahn YoungHwan,
Lee Gwang
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.966
Subject(s) - chemistry , lactic acid , chromatography , pyruvic acid , selected ion monitoring , mass spectrometry , ketone bodies , gas chromatography–mass spectrometry , ketone , selected reaction monitoring , lactic acidosis , carboxylic acid , tandem mass spectrometry , organic chemistry , biochemistry , metabolism , bacteria , genetics , biology
Simultaneous determination of lactic acid, pyruvic acid, 3‐hydroxybutyric acid and acetoacetic acid for clinical monitoring of lactic acidosis and ketone body formation in human plasma (20 µL) was performed by gas chromatography–mass spectrometry in selected ion monitoring (SIM) mode after generating methoxime/tert‐butyldimethylsilyl derivatives. All of the targeted carboxylic acids were detected by characteristic fragment ions, which permitted sensitive and selective identification in the presence of co‐extracted free fatty acids and other acidic metabolites at much higher levels. The method was linear ( r ≥ 0.9991), reproducible (% relative standard deviation = 1.2–5.8), and accurate (% relative error = −7.2–7.6), with detection limits of 0.05–1.7 ng/mL. This rapid, accurate and selective method using minimal plasma samples (20 µL) is useful in the clinical monitoring of lactic acidosis and ketone body formation in plasma. Copyright © 2008 John Wiley & Sons, Ltd.